Protein Details: Two-pore potassium channel 1

Protein ID

ICDB_Pro_1271

Protein Name

Two-pore potassium channel 1

Gene Name

TPK1; KCO1; At5g55630; MDF20.7

Organism

Arabidopsis thaliana (Mouse-ear cress)

Length

363 amino acids

AlphaFoldDB

AF-Q8LBL1-F1-model_v4.pdb

Function

Voltage-independent, large conductance and potassium-selective tonoplast ion channel. Regulated by cytoplasmic calcium and pH. Does not mediate slow-vacuolar (SV) ionic currents, but essential to establish VK currents. Has some permeability for Rb+ and NH4+, but none for Na+, Cs+ or Li+. Involved in intracellular K+ redistribution and/or K+ retranslocation between different tissues.

Sequence

MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQTTSQIQR

PDB Structures

Ligand Binding

1. DICL_CP

2. DICL_Pep

Binding Site

301;303;305;312;340;342;344;346;351

Disease

Location

Detected in mesophyll cells; guard cells and vascular tissues of the leaves. Expressed in the hilum; where the funiculus is attached during fruit maturation and in the embryo. Also expressed at a lower level in seedlings; root tips and elongation zones; and flowers. Could be detected in mitotically active tissues.

DOI ID

10.1093/emboj/16.10.2565; 10.1046/j.1365-313x.2002.01260.x; 10.1093/dnares/5.1.41; 10.1111/tpj.13415; 10.1104/pp.126.4.1646; 10.1016/s0014-5793(01)03273-2; 10.1073/pnas.0401502101; 10.1104/pp.105.065599; 10.1111/j.1365-313x.2006.02868.x; 10.1111/j.1365-313x.2007.03255.x; 10.1073/pnas.0702595104; 10.1093/mp/ssn064; 10.1104/pp.111.177816

RefSeq

NP_200374.1; NP_851196.1